hegf (Miltenyi Biotec)

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Miltenyi Biotec hegf
Hegf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hegf/Human+EGF%2C+premium+grade/pmc12700128-128-7-15
Average 95 stars, based on 68 article reviews

hegf - by Bioz Stars, 2026-09

95/100 stars

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Recombinant:

Article Title: Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells
Article Snippet: .. Always keep Matrigel on ice until coating. hEGF (Human recombinant epithelial growth factor, premium grade, Miltenyi Biotec, cat. no. 130‐097‐751). ..

Incubation:

Article Title: Targeting pregnane X receptor with a potent agonist-based PROTAC to delay colon cancer relapse.
Article Snippet: To cultivate tumoroids, 1000 cells were seeded on 96-well plates at a density of 1000 cells in 40 μL of a DMEM/Matrigel mixture (v:v) (Corning). .. The plates were incubated at 37 °C for 20min to allow dome formation, after which the domes were overlaid with 100 μL of DMEM/F12 medium (Thermo Fisher Scientific) supplemented with Supplement N2 (Thermo Fisher Scientific), hEGF (Miltenyi), hFGF (Miltenyi), 0.3% glucose, and 0.002% insulin (Sigma). .. Two days post-seeding, cells were treated with or without PROTACs (5 μM final concentration) for 16 hours and then exposed to increasing concentrations of chemotherapies for 72 h. Drug toxicity in the tumoroids was assessed using the CellTiter-Glow 3D viability assay (Promega), following the manufacturer’s instructions.

Cell Culture:

Article Title: Extraembryonic mesoderm cells derived from human embryonic stem cells rely on Wnt pathway activation.
Article Snippet: Other antibodies and reagents used for flow cytometry analysis included the anti-CDH1 antibodies (rat IgG1 APC isotype control; Table S1), hMSC-negative cocktail (isotype: PE hMSC isotype control negative cocktail), antiCD44 (isotype: PE mouse IgG2b isotype control) and hMSC-positive cocktail (CD73-APC, CD90-FITC, CD105-PerCP, isotype: hMSCpositive isotype control cocktail; BD, cat# 562245). .. The sorted EXMCsLUM+ and EXMCsBST2+ were cultured in medium consisting of DMEM/F12, 0.3% BSA (Sigma-Aldrich, A3059, St. Louis, MO, USA), 0.2% FBS, 1% ITS-X (100x, Gibco, 51,500,056), 1.5 mg/ mL L-ascorbic acid (Sigma-Aldrich, A8960-5G), 0.5 mM A83-01 (Selleck, s7692), 1 mM SB431542 (Cellagentec, 3814, San Diego, CA, USA), 50 ng/mL hEGF (Miltenyi Biotec, 130–097-750, Bergisch Gladbach, Germany), 2 μM CHIR99021, 0.8 mM valproic acid (SigmaAldrich, V0033000), 0.1 mM beta-mercaptoethanol (Sigma-Aldrich, M3148-25ML) and 5 mM Y-2763214 at 37 C and 5% CO2 with saturated humidity. .. After reaching 80%–90% confluence, the cells were disassociated into single cells using the TrypLE treatment for 5 min at 37 C. The cells were routinely passaged and re-plated on the Petri dishes (Corning, 430,166) precoated with 1% human collagen (SigmaAldrich, C7521-5MG) at a 1:3 split ratio every 3–4 days.

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